Nitrogen fixation capacity and metabolite responses to phosphorus in soybean nodules

Abstract Phosphorus (P) is necessary for nitrogen fixation in the root nodules of soybeans, a symbiotic process whereby plants support bacterial to obtain needed plant growth. Nitrogen accumulation, quantity, weight, specific nitrogenase activity (SNA) and acetylene reduction (ARA) soybean were analyzed, using broadly targeted metabolomics method incorporating liquid chromatography-mass spectrometry (LC-MS) study effects P level (1, 11, 31, 61 mg/L denoted by P1, P11, P31, P61) on types abundance various metabolites expression associated metabolic pathways nodules. SNA ARA inhibited stress. Kyoto Encyclopedia Genes Genomes (KEGG) analysis indicated that responded stress increasing number amino acids derivatives. Down-regulation ABA, phosphorylcholine, D-glucose 6-phosphate affected carotenoid biosynthesis, glycerophospholipid metabolism sugar which nodule under More flavonoids involved processes regulated nodulation fixation. The pathway ascorbate aldarate metabolism, metabolites, adaptation system starvation. This work provides foundation future investigations physiological mechanisms underly phosphorus soybeans.